![]() ![]() For transformation, the relatively new technique of gene bombardment is fast becoming a popular alternative because transgenes are often integrated into the genome at low copy number, which offers a number of advantages as described below ( Praitis et al., 2001 Berezikov et al., 2004). ![]() Moreover, microinjection is a very effective approach to RNA interference (see Reverse genetics), and can be used to deliver synthetic mRNAs or other molecules directly to cells ( Kimble et al., 1982 Bossinger and Schierenberg, 1992 Evans et al., 1994). Microinjection is a proven and relatively simple method for introducing DNA into worms ( Mello et al., 1991 Mello and Fire, 1995). ![]() However, homologous recombination into endogenous loci can occur at low frequency suggesting that targeted gene replacement is possible ( Broverman et al., 1993 Berezikov et al., 2004). Current transformation techniques generate large extrachromosomal DNA arrays, or cause "random" integration of transgenes into the genome. Furthermore, transgenes can be powerful tools for the design of new genetic screens. Transformation is used to clone genes by mutant rescue, to over-express or ectopically express genes, to express tagged proteins, to study structure/function of protein domains, and to analyze DNA or RNA regulatory elements. Continued abuse of our services will cause your IP address to be blocked indefinitely.DNA transformation and microinjection are essential tools for C. Please fill out the CAPTCHA below and then click the button to indicate that you agree to these terms. If you wish to be unblocked, you must agree that you will take immediate steps to rectify this issue. If you do not understand what is causing this behavior, please contact us here. If you promise to stop (by clicking the Agree button below), we'll unblock your connection for now, but we will immediately re-block it if we detect additional bad behavior. Overusing our search engine with a very large number of searches in a very short amount of time.Using a badly configured (or badly written) browser add-on for blocking content.Running a "scraper" or "downloader" program that either does not identify itself or uses fake headers to elude detection.Using a script or add-on that scans GameFAQs for box and screen images (such as an emulator front-end), while overloading our search engine.There is no official GameFAQs app, and we do not support nor have any contact with the makers of these unofficial apps. Continued use of these apps may cause your IP to be blocked indefinitely. This triggers our anti-spambot measures, which are designed to stop automated systems from flooding the site with traffic. Some unofficial phone apps appear to be using GameFAQs as a back-end, but they do not behave like a real web browser does.Using GameFAQs regularly with these browsers can cause temporary and even permanent IP blocks due to these additional requests. If you are using the Brave browser, or have installed the Ghostery add-on, these programs send extra traffic to our servers for every page on the site that you browse, then send that data back to a third party, essentially spying on your browsing habits.We strongly recommend you stop using this browser until this problem is corrected. The latest version of the Opera browser sends multiple invalid requests to our servers for every page you visit.The most common causes of this issue are: Your IP address has been temporarily blocked due to a large number of HTTP requests. ![]()
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